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Interference Microscopy for Cellular Studies
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Author(s): Alexey R. Brazhe (Technical University of Denmark, Denmark and Moscow State University, Russia), Nadezda A. Brazhe (Technical University of Denmark, Denmark and Moscow State University, Russia), Alexey N. Pavlov (Saratov State University, Russia)and Georgy V. Maksimov (Moscow State University, Russia)
Copyright: 2009
Pages: 17
Source title:
Handbook of Research on Systems Biology Applications in Medicine
Source Author(s)/Editor(s): Andriani Daskalaki (Max Planck Institute for Molecular Genetics, Germany)
DOI: 10.4018/978-1-60566-076-9.ch037
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Abstract
This chapter describes the application of interference microscopy and double-wavelet analysis to noninvasive study of cell structure and function. We present different techniques of phase and interference microscopy and discuss how variations in the intrinsic optical properties of a cell can be related to the intracellular processes. Particular emphasis is given to the newly developed phase modulation laser interference microscope. We show how this setup, combined with wavelet analysis of the obtained data series, can be applied to live cell imaging to investigate the rhythmic intracellular processes and their mutual interactions. We hope that the discussion will contribute to the understanding and learning of new methods for non-invasive investigation of intracellular processes.
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