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Personal Diagnostics Using DNA-Sequencing
Abstract
DNA sequencing is the process to identification of nucleotides order in genome which developed from very broad history, also it is derived from version of the Sanger biochemistry. SOLiD, 454 and Polonator sequencing based on emulsion PCR to amplify clonal sequencing with in-vitro construction of adaptor-flanked shotgun library, PCR amplified in the context of a water-in-oil emulsion. Solexa technology relies on bridge PCR to amplify clonal sequencing features. At the conclusion of the PCR, each clonal cluster contains ~1,000 copies of a single member of the template library. This chapter focused on next-generation sequencing technologies methods, capabilities and clinical applications of DNA sequencing technologies for researchers in molecular biology and physician scientists. This will also provide the power of these novel genomic tools and methods to use personal diagnostic at molecular level.
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