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Automated Bacterial Colony Counting for Clonogenic Assay

Automated Bacterial Colony Counting for Clonogenic Assay
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Author(s): Wei-Bang Chen (University of Alabama at Birmingham, USA)and Chengcui Zhang (University of Alabama at Birmingham, USA)
Copyright: 2009
Pages: 12
Source title: Dental Computing and Applications: Advanced Techniques for Clinical Dentistry
Source Author(s)/Editor(s): Andriani Daskalaki (Max Planck Institute for Molecular Genetics, Germany)
DOI: 10.4018/978-1-60566-292-3.ch009

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Abstract

Bacterial colony enumeration is an essential tool for many widely used biomedical assays. This chapter introduces a cost-effective and fully automatic bacterial colony counter which accepts digital images as its input. The proposed counter can handle variously shaped dishes/plates, recognize chromatic and achromatic images, and process both color and clear medium. In particular, the counter can detect dish/plate regions, identify colonies, separate aggregated colonies, and finally report consistent and accurate counting result. The authors hope that understanding the complicated and labor-intensive nature of colony counting will assist researchers in a better understanding of the problems posed and the need to automate this process from a software point of view, without relying too much on specific hardware.

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