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Application of Phage Biotechnology in Nanobiotechnology
Abstract
To date, the phage display system has enabled the discovery of material binding peptides. Diversity and functionality of these peptides could be improved using RNA-based display systems instead of the conventional DNA-based ones. RNA phage replication systems possess unique features that make them a versatile tool for any combinatory approach and evolutionary application. Phage display was used to monitor the chemical surface properties and to initiate nanoparticle assembly. Novel bio-panning was recently used in RNA-based display to screen new functionality without acidic elution used in other conventional DNA phage display systems. Therefore, Hybrid RNA phages would be a perfect platform for attachment and exploration of nanoparticles. In this chapter, the authors present an overview on research conducted on these cross fields and areas. They not only focus on the novel selection and amplification process but also on the importance of RNA phage and its peptide display as tools for preventing nanoparticle aggregation.
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